KOH test

KOH test

The KOH test is a procedure in which potassium hydroxide (KOH) is used to detect fungi by dissolving human cells in a culture. The difference in cell wall composition of human cells and fungal cells allows this procedure to help distinguish the two cells. KOH denatures the proteins in the human cell; only the fungal cells remain to be seen under the microscope.

KOH test procedure:

# Take scraping from margin (not center) of lesion
# Place on clean slide
# Add 2-3 drops of 10% KOH in water
# Warm the slide (don't boil)
# Optional - add 1 drop of lactophenol cotton blue
# Add cover slip
# Examine immediately under high dry magnification with light microscope

It is used to detect whether a patient has Athlete's foot and many other fungal infections.


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